Microscopists at the Weld Hill Research Facilities will find a range of tools from the simple hand lens to the confocal microscope for 3-D reconstructions. Registered and trained users of the facilities may login to Booked to reserve equipment.
Microscopes
JEOL JSM-6010 LV Scanning Electron Microscope Resolution: 4.0 nm in HV mode; 5.0 nm in LV mode Magnification: 80X to 300kX Accelerating Voltage: 500 V to 20 kV Detectors: Everhart Thornley, Backscatter with COMPO, TOPO, and Shadow, Low Vacuum Secondary Electron detector Operating Pressure: 10-4 Pa order to 100Pa Other: motorized 2 axis stage, chamberscope, stage navigation system | Zeiss LSM900 Confocal Microscope Microscope: Axioimager.Z2 Objectives: 5x/0.16 EC Plan-Neofluar; 10x/0.45 (DIC) Plan-Apochromat; 20x/0.8 (DIC) Plan-Apochromat; 20x/0.5 W N-Achroplan (Water dipping, 2.6 mm working distance); 40x/1.2 Water (DIC) C-Apochromat W; 63x/1.2 Water (DIC) C-Apochromat W; 100x/1.46 Oil (DIC) alpha-Plan-Apochromat Confocal and Widefield Imaging Software: Zen Blue Confocal Laser Lines: 405nm, 488nm, 561nm, 640nm Confocal Detectors: Confocal PMT and Airyscan Widefield Imaging Camera: AxioCam 712 Fluorescent Light Source: Colibri 7 Type R[G/Y]CBV-UV Fluorescent Filters: Position 1: DAPI, GFP, RFP, Cy5 (90 HE LED); Position 2: CFP, YFP, mCherry (91 HE LED) | Zeiss Axioimager (Auto) Upright Microscope Microscope: Axioimager.Z2 Objectives: 2.5x/0.075 EC Plan-Neofluar 10x/0.45 (DIC) Plan-Apochromat; 20x/0.8 (DIC) Plan-Apochromat; 40x/0.95 Korr (DIC) Plan-Apochromat; 40x/1.1 Water (DIC) LD C-Apochromat W; 63x/1.40 Oil (DIC) Plan-Apochromat Widefield Imaging Software: Zen Blue 2.3 (multichannel, Z-stack, tiling, time lapse) Camera: AxioCam 512 Fluorescent Light Source: Lumencor SOLA-365-SE light engine Fluorescent Filters: Position 1: DAPI narrow (Chroma Custom set) excitation G 365/10; dichroic mirror T 387 LP; emission 400 LP; Position 2: DAPI wide (Zeiss Filter set 49, 488049-0000) excitation G 365; beamsplitter FT 395; emission BP 445/50; Position 3: FITC/AF488/GFP minus chlorophyll auto-fluorescence (Chroma filter set 49002) excitation BP 470/40; beamsplitter T 495; emission BP 525/50 Position 4: Rhodamine (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640 |
Zeiss AxioImager (Manual) Upright Microscope Microscope: Axioimager.A2 Objectives: 5x/0.16 EC Plan-Neofluar; 10x/0.45 (DIC) Plan-Apochromat; 20x/0.8 (DIC) Plan-Apochromat; 40x/0.95 Korr (DIC) Plan-Apochromat; 40x/1.3 Plan Neofluar Oil 63x/1.40 (DIC) Plan-Apochromat Oil Camera: AxioCam 512 Software: Zen Blue Pro with multichannel Fluorescent Light Source: Lumencor SOLA-365-SE light engine Fluorescent Filters: Position 1: DAPI narrow (Chroma Custom set) excitation G 365/10; dichroic mirror T 387 LP; emission 400 LP; Position 2: FITC/AF488/GFP plus chlorophyll auto-fluorescence (Chroma 49012) excitation: BP 480/40; beamsplitter: FT 510; emission: 510 LP Position 3: FITC/AF488/GFP minus chlorophyll auto-fluorescence (Zeiss Filter set 38, 1031-346) excitation BP 470/40; beamsplitter FT 495; emission BP 525/50; Position 4: Rhodamine (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640 | Zeiss Axiophot Upright Microscope Microscope: Axiophot Objectives: 5x/0.15 Plan-Neofluar; 10x/0.30 (DIC) Plan-Neofluar Pol; 20x/0.5 (DIC) Plan-Neofluar Pol; 40x/0.85 (DIC) Plan-Neofluar Pol; 63x/1.4 (DIC) Plan-Apochromat Oil Camera: AxioCam 512 Software: Zen Blue with multichannel Fluorescent Light Source: Lumencor SOLA-365-SE light engine Fluorescent Filters: Position I: DAPI narrow (Zeiss 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397; Position II: DAPI wide (Chroma 49000) excitation G 350/50; dichroic mirror FT 400; barrier filter 460/50; Position III: AF488/FITC/GFP plus chlorophyll auto-fluorescence (Zeiss Filter set 9) excitation 450-490; dichroic mirror 510; emission filter LP515; Position IV: Rhodamine (Zeiss Filter set 15) excitation 540-560 nm; dichroic mirror FT 580; barrier filter LP590 | Zeiss Axioskop Upright Microscope Microscope: Axioskop Objectives: 2.5x/0.075 Plan-Neofluar; 10x/0.30 (DIC) Plan-Neofluar Ph1; 20x/0.5 (DIC) Plan-Neofluar Ph2; 40x/0.75 (DIC) Plan-Neofluar Ph2 Camera: AxioCam HRc Software: Zen Blue with multichannel Fluorescent Light Source: Lumencor SOLA-365-SM light engine Fluorescent Filters: Position I: DAPI narrow (Zeiss 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397; Position II: Rhodamine (Zeiss Filter set 15) excitation 540-560 nm; dichroic mirror FT 580; barrier filter LP590; Position III: AF488/FITC/GFP plus chlorophyll auto-fluorescence (Zeiss Filter set 9) excitation 450-490; dichroic mirror 510; emission filter LP515 |
Zeiss Microspectrophotometer Microspectrophotometer: MSP20 Objectives: 10x/0.30 Plan-Neofluar; 20x/0.5 Plan-Neofluar; 40x/0.75 Plan-Neofluar Pol; 100x/1.3 Plan-Neofluar Oil; 100x/1.3 Plan-Neofluar Oil Iris Fluorescent Light Source: HBO 100-W burner Fluorescent Filters: DAPI (Zeiss Filter set 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397 | Zeiss Discovery Dissecting Microscope Microscope: Discovery v12 Objectives: 0.63x PlanApo S; 1.5x PlanApo S; 2.3x PlanApo S Camera: AxioCam 512 Software: Zen Blue Pro with extended focus and time lapse modules | Zeiss Stemi 11 Dissecting Microscope Microscope: Stemi sv11 Objectives: 1.0x Plan Apochromat S; 1.6x Plan S Camera: AxioCam HRc Software: Zen Blue with time lapse |
Zeiss Stemi 2000-C Dissecting Microscope Microscope: Stemi 2000-C Objectives: 1.0x Plan Apochromat S; 1.6x Plan S | Zeiss Stemi 508 Dissecting Microscope Microscope: Stemi 508 DocObjectives: 0.63x Apo;1.0x glass Camera: Axiocam 208 Color Software: Stand Alone On-Screen Display (OSD) Light Source: KL 300 LED with ring illuminator Location: Weld Hill Growth Facilities | NightSea Fluorescence Adapters for dissecting scopes Filter Sets: 1) UV – Ultraviolet (DAPI, Aniline Blue): excitation 360-380nm; emission 415nm LP 2) RB-GO – Green Only (EGFP minus autofluorescence): excitation 440-460nm; emission 500-560nm BP 3) RB – Royal Blue (EGFP plus autofluorescence): excitation 440-460nm; emission 500nm LP 4) CY – Cyan (YFP plus autofluorescence): excitation 490-515nm; emission 500nm LP 5) GR – Green (DsRed, dTomato): excitation 510-540nm; emission 600nm LP |
Other Microscopy Tools
| Tousimis Autosamdri-815 Series A Critical Point Dryer  | Denton Vacuum Desk II Sputter Coater  | BP-111-RS Lab Microwave Microwave processing of tissue  Care of microwave Microwave fixation for RNA Microwave processing and immunolocalization microwave mounting of slide |
| Memmert Paraffin Oven UN30pa Paraffin oven  | TBS Embedding Station Care of embedding center Embedding | Rotary Microtome Thick paraffin sections  |
| Leica RM2155 Microtome Thick resin sections  Leica_RM2155 Manual | Thermo Scientific HM360 Microtome Thick resin sections  | RMC MT-7 Ultra Microtome Ultra thin resin sections  |
| TPI Vibratome 1000/2000 Fresh sections  | Reichert-Jung 2800 Frigocut E Frozen sections  | Leica SM 2010R Sliding microtome  |