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1927 Map of the Arboretum

Weld Hill Microscopy Lab

Prunus incisa early stage ovule. Image taken with a upright microscope. Jorge Lora (2013)
Prunus incisa

Microscopists at the Weld Hill Research Facilities will find a range of tools from the simple hand lens to the confocal microscope for 3-D reconstructions. Registered and trained users of the facilities may login to Booked to reserve equipment.

Microscopes

JEOL JSM-6010 LV Scanning Electron Microscope

scanning electron microscope

Resolution: 4.0 nm in HV mode; 5.0 nm in LV mode
Magnification: 80X to 300kX
Accelerating Voltage: 500 V to 20 kV
Detectors: Everhart Thornley, Backscatter with COMPO, TOPO, and Shadow, Low Vacuum Secondary Electron detector
Operating Pressure: 10-4 Pa order to 100Pa
Other: motorized 2 axis stage, chamberscope, stage navigation system
Zeiss LSM900 Confocal Microscope

confocal microscope
Microscope: Axioimager.Z2
Objectives: 5x/0.16 EC Plan-Neofluar;
10x/0.45 (DIC) Plan-Apochromat;
20x/0.8 (DIC) Plan-Apochromat;
20x/0.5 W N-Achroplan (Water dipping, 2.6 mm working distance);
40x/1.2 Water (DIC) C-Apochromat W;
63x/1.2 Water (DIC) C-Apochromat W;
100x/1.46 Oil (DIC) alpha-Plan-Apochromat
Confocal and Widefield Imaging Software: Zen Blue
Confocal Laser Lines: 405nm, 488nm, 561nm, 640nm
Confocal Detectors: Confocal PMT and Airyscan
Widefield Imaging Camera: AxioCam 712
Fluorescent Light Source: Colibri 7 Type R[G/Y]CBV-UV
Fluorescent Filters:
Position 1: DAPI, GFP, RFP, Cy5 (90 HE LED);
Position 2: CFP, YFP, mCherry (91 HE LED)
Zeiss Axioimager (Auto) Upright Microscope



Microscope: Axioimager.Z2
Objectives: 2.5x/0.075 EC Plan-Neofluar
10x/0.45 (DIC) Plan-Apochromat;
20x/0.8 (DIC) Plan-Apochromat;
40x/0.95 Korr (DIC) Plan-Apochromat;
40x/1.1 Water (DIC) LD C-Apochromat W;
63x/1.40 Oil (DIC) Plan-Apochromat
Widefield Imaging Software: Zen Blue 2.3 (multichannel, Z-stack, tiling, time lapse)
Camera: AxioCam 512
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position 1: DAPI narrow (Chroma Custom set) excitation G 365/10; dichroic mirror T 387 LP; emission 400 LP;
Position 2: DAPI wide (Zeiss Filter set 49, 488049-0000) excitation G 365; beamsplitter FT 395; emission BP 445/50;
Position 3: FITC/AF488/GFP minus chlorophyll auto-fluorescence (Chroma filter set 49002) excitation BP 470/40; beamsplitter T 495; emission BP 525/50
Position 4: Rhodamine (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640
Zeiss AxioImager (Manual) Upright Microscope

Axioimager microscope

Microscope: Axioimager.A2
Objectives: 5x/0.16 EC Plan-Neofluar;
10x/0.45 (DIC) Plan-Apochromat;
20x/0.8 (DIC) Plan-Apochromat;
40x/0.95 Korr (DIC) Plan-Apochromat;
40x/1.3 Plan Neofluar Oil
63x/1.40 (DIC) Plan-Apochromat Oil
Camera: AxioCam 512
Software: Zen Blue Pro with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position 1: DAPI narrow (Chroma Custom set) excitation G 365/10; dichroic mirror T 387 LP; emission 400 LP;
Position 2: FITC/AF488/GFP plus chlorophyll auto-fluorescence (Chroma 49012) excitation: BP 480/40; beamsplitter: FT 510; emission: 510 LP
Position 3: FITC/AF488/GFP minus chlorophyll auto-fluorescence (Zeiss Filter set 38, 1031-346) excitation BP 470/40; beamsplitter FT 495; emission BP 525/50;
Position 4: Rhodamine (Zeiss Filter set 20, 488020-0000) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640
Zeiss Axiophot Upright Microscope


Microscope: Axiophot
Objectives: 5x/0.15 Plan-Neofluar;
10x/0.30 (DIC) Plan-Neofluar Pol;
20x/0.5 (DIC) Plan-Neofluar Pol;
40x/0.85 (DIC) Plan-Neofluar Pol;
63x/1.4 (DIC) Plan-Apochromat Oil
Camera: AxioCam 512
Software: Zen Blue with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SE light engine
Fluorescent Filters:
Position I: DAPI narrow (Zeiss 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397;
Position II: DAPI wide (Chroma 49000) excitation G 350/50; dichroic mirror FT 400; barrier filter 460/50;
Position III: AF488/FITC/GFP plus chlorophyll auto-fluorescence (Zeiss Filter set 9) excitation 450-490; dichroic mirror 510; emission filter LP515;
Position IV: Rhodamine (Zeiss Filter set 15) excitation  540-560 nm; dichroic mirror FT 580; barrier filter LP590
Zeiss Axioskop Upright Microscope

upright microscope
Microscope: Axioskop
Objectives: 2.5x/0.075 Plan-Neofluar;
10x/0.30 (DIC) Plan-Neofluar Ph1;
20x/0.5 (DIC) Plan-Neofluar Ph2;
40x/0.75 (DIC) Plan-Neofluar Ph2
Camera: AxioCam HRc
Software: Zen Blue with multichannel
Fluorescent Light Source: Lumencor SOLA-365-SM light engine
Fluorescent Filters:
Position I: DAPI narrow (Zeiss 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397;
Position II: Rhodamine (Zeiss Filter set 15) excitation  540-560 nm; dichroic mirror FT 580; barrier filter LP590;
Position III: AF488/FITC/GFP plus chlorophyll auto-fluorescence (Zeiss Filter set 9) excitation 450-490; dichroic mirror 510; emission filter LP515
Zeiss Microspectrophotometer

microspectrophotometer
Microspectrophotometer: MSP20
Objectives
: 10x/0.30 Plan-Neofluar;
20x/0.5 Plan-Neofluar;
40x/0.75 Plan-Neofluar Pol;
100x/1.3 Plan-Neofluar Oil;
100x/1.3 Plan-Neofluar Oil Iris
Fluorescent Light Source: HBO 100-W burner
Fluorescent Filters: DAPI (Zeiss Filter set 48702) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397
Zeiss Discovery Dissecting Microscope


Microscope: Discovery v12
Objectives: 0.63x PlanApo S; 1.5x PlanApo S; 2.3x PlanApo S
Camera: AxioCam 512
Software: Zen Blue Pro with extended focus and time lapse modules
Zeiss Stemi 11 Dissecting Microscope


Microscope: Stemi sv11
Objectives:
1.0x Plan Apochromat S;
1.6x Plan S
Camera: AxioCam HRc
Software: Zen Blue with time lapse
Zeiss Stemi 2000-C Dissecting Microscope

dissecting microscope
Microscope: Stemi 2000-C
Objectives: 1.0x Plan Apochromat S;
1.6x Plan S
Zeiss Stemi 508 Dissecting Microscope

dissecting scopeMicroscope: Stemi 508 Doc
Objectives:
0.63x Apo;1.0x glass
Camera: Axiocam 208 Color
Software: Stand Alone On-Screen Display (OSD)
Light Source: KL 300 LED with ring illuminator
Location: Weld Hill Growth Facilities

NightSea Fluorescence Adapters for dissecting scopes

Filter Sets:
1) UV – Ultraviolet (DAPI, Aniline Blue): excitation 360-380nm; emission 415nm LP
2) RB-GO – Green Only (EGFP minus autofluorescence): excitation 440-460nm; emission 500-560nm BP
3) RB – Royal Blue (EGFP plus autofluorescence): excitation 440-460nm; emission 500nm LP
4) CY – Cyan (YFP plus autofluorescence): excitation 490-515nm; emission 500nm LP
5) GR – Green (DsRed, dTomato): excitation 510-540nm; emission 600nm LP

Other Microscopy Tools

Tousimis Autosamdri-815 Series A
Critical Point Dryer

Denton Vacuum Desk II
Sputter Coater

sputter coater
BP-111-RS Lab Microwave
Microwave processing of tissue

Lab-microwave
Care of microwave
Microwave fixation for RNA
Microwave processing and immunolocalization
microwave mounting of slide
Memmert Paraffin Oven UN30pa
Paraffin oven

TBS Embedding Station
Care of embedding center
Embedding
Rotary Microtome
Thick paraffin sections

paraffin microtome
Leica RM2155 Microtome
Thick resin sections

resin microtome
Leica_RM2155 Manual
Thermo Scientific HM360 Microtome
Thick resin sections

resin microtome
RMC MT-7 Ultra Microtome
Ultra thin resin sections

TPI Vibratome 1000/2000
Fresh sections

Reichert-Jung 2800 Frigocut E
Frozen sections

Leica SM 2010R
Sliding microtome

sliding microtome

More Microscopy Protocols

Feulgen stainingSectioning 101