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Arnold Arboretum

Weld Hill Microscopy Lab

Microscopists at Weld Hill will find a range of tools from the simple hand lens to the confocal microscope for 3-D reconstructions.

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JEOL JSM-6010 LV Scanning Electron Microscope

Resolution: 4.0 nm in HV mode; 5.0 nm in LV mode
Magnification: 80X to 300kX
Accelerating Voltage: 500 V to 20 kV
Detectors: Everhart Thornley, Backscatter with COMPO, TOPO, and Shadow, Low Vacuum Secondary Electron detector
Operating Pressure: 10-4 Pa order to 100Pa
Other: motorized 2 axis stage, chamberscope, stage navigation system

Zeiss LSM700 Confocal Microscope

Objectives: 2.5x/0.075 M27  Plan-Neofluar; 5x/0.16 M27  Plan-Neofluar;10x/0.45 M27  Plan-Apochromat;20x/0.8 M27  Plan-Apochromat; 40x/0.95 Corr M27  Plan-Apochromat; 63x/1.40 Oil DIC M27 Plan-Apochromat; 100x/1.46 Oil DIC M27 Plan-Apochromat
Camera: AxioCam HRc
Software: Confocal -  Zen 2010; Brightfield/fluorescence – AxioVision 4 with mosaiX and Z-Stack modules
Laser Lines: 405nm, 488nm, 555nm, 639nm
Fluorescent Light Source: HBO 100-W
Fluorescent Filters: Filter set 20 (488020-0000) (Rhodamin) excitation BP 546/12; beamsplitter FT 560; emission BP 575-640; Filter set 38 (1031-346) (Endow GFP) excitation BP 470/40; beamsplitter FT 495; emission BP 525/50; Filter set 49 (488049-0000) (DAPI) excitation G 365; beamsplitter FT 395; emission BP 445/50

Zeiss Discovery v12 Dissecting microscope

Objectives: 0.63x PlanApo S; 1.5x PlanApo S; 2.3x PlanApo S
Camera: AxioCam HRc
Software: AxioVision 4 with extended focus and time lapse modules

Zeiss AxioImager A2 Upright microscope

Objectives: 10x/0.45 Plan-Apochromat; 20x/0.8 Plan-Apochromat; 40x/0.95 Plan-Apochromat; 63x/1.40 Oil Plan-Apochromat
Camera: AxioCam HRc
Software: AxioVision 4

Zeiss Axiophot

Microscope: Zeiss Axiophot
Objectives: 2.5x/0.075 Plan Neofluar; 10x/0.30 Pol Plan-Neofluar; 20x/0.5 Pol Plan-Neofluar; 40x/0.85 Pol Plan-Neofluar Pol; 63x/1.4 Oil DIC Plan-Apochromat
Camera: AxioCam MRc
Software: AxioVision 4
Fluorescent Light Source: HBO 100-W
Fluorescent Filters: Filter set – 1 (48702, DAPI) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397; Filter set – 2 (487902, DAPI) excitation G 365; dichroic mirror FT 395; barrier filter LP420; Filter set – 3 (Red) excitation BP 530-585 nm; dichroic mirror FT 600; barrier filter LP615;

Zeiss Microspectrophotometer

Microspectrophotometer: Zeiss MSP 20
Microscope: Zeiss Axioskop
Objectives: 5x/0.15 Plan-Neofluar; 10x/0.30 Ph1 Plan-Neofluar; 20x/0.5 Ph2 Plan-Neofluar; 40x/0.75 Ph2 Plan-Neofluar
Fluorescent Light Source: HBO 100-W burner
Fluorescent Filters: Filter set 48702 (DAPI) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397

Zeiss Axioskop

Microscope: Zeiss Axioskop
Objectives: 5x/0.15 Plan-Neofluar; 10x/0.30 Ph1 Plan-Neofluar; 20x/0.5 Ph2 Plan-Neofluar; 40x/0.75 Ph2 Plan-Neofluar
Camera: None
Fluorescent Light Source: HBO 50-W
Fluorescent Filters: Filter set – 1 (48702, DAPI) excitation G 365, bandpass 12 nm; dichroic mirror FT 395; barrier filter LP397; Filter set – 2 (487902, DAPI) excitation G 365; dichroic mirror FT 395; barrier filter LP420; Filter set – 3 (Red) excitation BP 530-585 nm; dichroic mirror FT 600; barrier filter LP615;

Zeiss Stemi SV 11

Objectives: 1.0x Plan Apochromat S; 1.6x Plan S
Camera: AxioCam HRc
Software: AxioVision 4

Zeiss Stemi 2000-C

Objectives: 1.0x Plan Apochromat S; 1.6x Plan S
 

 

Hand Lens Magnifier

BP-111-RS Lab Microwave

Care of microwave

Microwave fixation for RNA; Microwave processing and immunolocalization

microwave mounting of slides

TBS Embedding Station

Care of embedding center

Embedding

Thermo Scientific HM360 Microtome (Resin)

Leica RM2155 Microtome (Resin)

Leica_RM2155 Manual

Microtome (Paraffin)

Sectioning 101

Reichert-Jung 2800 Frigocut E

RMC MT-7 Ultra Microtome

More Protocols

Feulgen staining

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